Why were my gBlocks not amplified while my samples were amplified well in the same run?

I did PCR to amplify BCR-ABL genes and used three gBlocks as my positive controls. I used MyTaq HS Red Mix from Bioline. My sample generated a band representing internal control but all the...

04 May 2017 4,176 2 View

Why my melting curve analysis results from same samples change in different PCR run?

I did COLD-PCR for JAK2 wildtype and mutant samples. The previous results were okay, the peaks were around 76-77 celcius. Now the peaks are around 77-78, I wonder what happened. My samples are...

02 March 2016 1,644 5 View

What primer is best to use for analyzing bacterial endophytes in sweet potato tuber?

I want to know the bacterial endophytes in sweet potato tuber using PCR-DGGE method but I'm still curious about the primer I should use. I haven't found other research using this method to analyse...

31 December 2014 9,757 1 View

Does v1-v3 primer of 16S rRNA also detect sweet potato mitochondria?

I'm planning to use v1-v3 primer to analyse bacterial endophytes in sweet potato tuber by PCR-DGGE method and I'm wondering if this primer could also detect other DNA despite my target. I'm...

31 December 2014 8,589 2 View

Is there any open-source software to do analysis of bands in the gel resulting from DGGE?

My goal in my research is to analyse the dynamics of bacterial community structures in water samples from a zero-water discharge system. I might not sequence all the bands so I need to know if...

10 November 2014 1,872 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

No title

02 March 2021 3,060 3 View