Dear All,
In most protocols who maturate neurons from IPSC-derivered NPCs (neuronal progenitor cells); they use NEAA in stemcell stage and NPCs induction phase, some of them use NEAA in NPC medium as well. However only a limited number of them using NEAA during neuronal maturation from NPCs.
Is there a spesific reason that we dont use NEAA during neuronal differentiation or is it just because initial paper was like that and people followed it?
Because it seems like NEAA are harmless and support cellular growth in all cell types.
thank you for answers
Yagiz
Thank you for the answer. May I ask if you can suggest me to read some of your papers to see the whole protocol?
Thank you again
Yagiz
For neural cell culture, it is not optimal to use certain amino acids.
For example, you really should not use neuroactive compounds such as glutamic acid, which can cause excitotoxicity, despite their clear advantage as an energy source. Aromatic amino acids may also exhibit some signaling effects.
Jason Hamlin Thank you very much for the answer. It helped me a lot to understand.
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