Liyuan Zhang

3 Questions 9 Answers 0 Followers

Questions related from Liyuan Zhang

Does anyone have experience with HEK293 suspension adaption experience?

Hello researchers, recently i am starting adapting hek293 adherent cell line to suspension culture. i tried direct adaption, which is accutase cells then seed them directly to F17 complete medium...

23 February 2023 8,047 0 View

Can cells recovering from low viability?

Hello guys I get a cryovial of HEK293 suspension cells which is low viability after thaw, like 37%. I culturing them in a t25 flask now, Will the viability getting high after cell revived? Is...

14 February 2023 1,669 9 View

Why i cannot find IPSCs colonies after sendai infected?

Hi Is there anyone who have the experience with IPSCs generation? I infected the PBMCs 60 days ago, but i still not find any IPSCs colonies, almost all of them just looks like differentiation. But...

22 June 2018 4,357 5 View