The large darker structures on the right and along the bottom is the cell nucleus. The central vacuole is likely a lysosome that appears to contain the remnants of another cell that was phagocytosed. The remaining cytoplasmic details are not clear at this magnification and may be the result of sub-optimal fixation. The black spots are fixation/staining artefacts.

Hello Mehakpreet Thind

Neutrophils have two distinctive morphological characteristics namely, the shape of their nucleus and their granules. The nucleus of neutrophil seen in the image seems to be bi-lobed. Neutrophils usually contain nucleus which is 2-5 lobes, and hence the alternative name of “polymorphonuclear” often given to these cells.

Unstimulated neutrophils exhibit a smooth round cell shape with uniform cytoplasmic granularity, whereas irregular cell shape, toxic granulations, and cytoplasmic vacuolization can be observed in trauma-induced neutrophil activation.

The neutrophil seen in the image seems to be activated showing irregular cell shape and cytoplasmic vacuolization. As mentioned by Thomas J Raub, the cytoplasmic details are not clear. The cell should contain granules clearly visible in the cytoplasm. If you observe the nucleus, you can see the highly condensed heterochromatin (dark) is neatly marginalized to the edge of the nucleus, only interrupted by euchromatic areas close to nuclear pores that mostly line the nuclear membrane. The brighter euchromatin is mostly seen in the center of the lobules.

Best.

Thank you so much for your answer. Malcolm Nobre Thomas J Raub