Experimentally I have found that the prepared curcumin pyrazole has more polyphenolic content compared to pure curcumin but could no explain the reason behind. Can anyone please help me with the explanation?
Did you use FCR for the TPC assay? Did you confirm that pyrazole is the pure and only product of the reaction? Can you say all the synthesis reagents are removed from the reaction pot and by using HPLC single response belong to pyrazole...What kind of approaches did you use to validate the conversion reaction was successful?
İsmail Emir Akyildiz Yes, I did use FCR. Column chromatography was done to purify the product and NMR analysis was done. we just looked for the presence of N-N and N-H bonds as well as the disappearance of some of the curcumin peaks
Something (co-eluent from column chromatography) in the final product must interfere with the assay due to its reducing activity. I can suggest applying the same post-synthesis purification procedure to curcumin. The final composition of the curcumin and pyrazole derivate should be the same for the TPC assay or at least a reagent blank for each sample (curcumin and derivate) must be prepared and blank subtraction should be performed. I do not think the pyrazole ring itself can contribute to overall TPC results (Similar compounds were investigated in literature for their potential interferences). It may alter and change the electron distribution through the molecule owing to introduced electronegativity which may pose more reducing activity feature to the molecule (just an assumption)...
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