Please can you answer my question I have an extract that I dried and made different concentrations to calculate the percentage of phenolic compounds using Gallic acid as a standard using the Folin reagent method. After I took all the readings and represented them graphically I have calculated x value and it was 27.834 microgram by this equation y = 0.0175x + 0.0029 R² = 0.9994, where y is the absorbance of my sample which was 0.49 . now I want a way to convert x value into mg/ml and then calculate the amount of phenolic content present in the extract, knowing that the weight of the extract is 15.748 grams. If possible, a clear method for all calculations... Many thanks in advance
You can simplify the expression by dividing 27.834 micrograms by 1000 to convert it to milligrams:
TPC=27.834/1000
TPC=0.027834 mg
To convert the absorbance value (x) obtained from your calibration curve into milligrams per milliliter (mg/mL), you can use the equation of your calibration curve:
y = 0.0175x + 0.0029
Given that the absorbance of your sample (y) is 0.49, you can rearrange the equation to solve for x:
x = y - 0.0029/0.0175
x = 0.49 - 0.0029/0.0175
x = 0.4871/0.0175
x ≈ 27.806
Now that you have the absorbance value (x) in micrograms per milliliter (µg/mL), you can convert it to milligrams per milliliter (mg/mL) by dividing by 1000:
x =27.806/1000
x =0.027806 mg/mL
Now, to calculate the total phenolic content (TPC) in milligrams per gram (mg/g) of the extract, you can use the following steps:
1. Calculate the total phenolic content (TPC) in milligrams per milliliter (mg/mL) of the extract:
TPC (mg/mL)= x
2. Calculate the total phenolic content (TPC) in milligrams per gram (mg/g) of the extract using the weight of the extract (15.748 grams):
TPC (mg/g)= TPC (mg/mL) x volume of extract used (mL) x 1/weight of extract (g)
Given that the volume of extract used is typically 1 mL, the calculation simplifies to:
TPC (mg/g)= TPC (mg/mL) x 1/weight of extract (g)
Substitute the calculated TPC (0.027806 mg/mL) and the weight of the extract (15.748 grams) into the equation:
TPC (mg/g) = 0.027806 x 1 / 15.748
TPC (mg/g) = 0.001767 mg/g
So, the total phenolic content in your extract is approximately 0.001767 mg/g of Gallic Acid Equivalents (GAE).
NOTE= Please recheck for avoiding any mistake
- Badges
- Science topic
- Similar topics
- Agricultural Science
- Agronomy
- Crop Science