21 April 2024 1 290 Report

I am trying to purify protein extracts but polyphenols are covalently bound with proteins during roasting. Is there any method to remove covalently-bound polyphenols and purify proteins? Thank you!

More Yuxing Ge's questions See All
How to analyze the percentages of covalently-bound and noncovalently-bound polyphenols during protein extraction?

I extracted protein from a roasted food sample using alkaline extraction followed by acid precipitation. Polyphenols were always coextracted since they were covalently bound with proteins during...

01 May 2024 7,192 2 View

Using EGFP fusion protein positioning, it was found that there are linear luminophores in the hyphae of Fusarium. What subcellular structure is this?

Using EGFP fusion protein positioning, it was found that there are linear luminophores in the hyphae of Fusarium. What subcellular structure is this?

12 January 2024 4,557 0 View

Are there any specific protocols/guidelines to degass sodium hypochlorite using a vacuum pump, in terms of time and pressure?

Are there any specific protocols/guidelines to degass sodium hypochlorite using a vacuum pump, in terms of time and pressure? Or, are we relying on the visible absence of bubbles? Attempting to...

26 September 2023 3,044 0 View

How to improve the purity of my protein(see the figure below)?

The protein was purified with Ni column, and I got complete results...

17 October 2022 898 2 View

How to interpret a patch-clamp result?

Hi I'm new to electrophysiology and have been reading some papers. But i can't really understand how to read their results. For example, attached here is a screenshot from a paper (Hypoxia reduces...

02 February 2022 3,740 4 View

How to find centroid value of a doublet peak in Origin or GraphPad?

Hi! As shown in the following figure, there is a doublet peak in mass spectrum. I would like to find centroid value of this peak using an unbiased method in Origin, GraphPad or any other...

03 November 2021 7,247 8 View

How to deal with initialization bias from a simulation model?

Suppose we have a system designed to deliver services to customers arriving during weekdays. The arrival process is modeled as a Poisson process with an Arrival rate of λ, also we use agent-based...

23 August 2021 4,755 3 View

How to choose energy model and achieve it?

Dear scholar: I'm doing my doctoral thesis that 'Research on the Optimal Path and Countermeasures of China's Energy Transition Under the Constraints of Carbon Neutrality and Carbon Peak'.This...

08 July 2021 1,821 3 View

Why use KLAAALE as the linker peptide on C-terminus with his tag?

I have a plasmid that I inherited from a previous lab mate. It is his6-thrombin-POI-KLAAALE-his6. I couldn't figure out why KLAAALE is needed as the linker peptide on the C-terminus but I saw...

12 April 2021 8,930 5 View

Why cannot my FH primary antibody recognize the exogenous expressed Flag-tagged FH protein from 293T cells?

I overexpressed Flag-tagged FH (fumarase) by transfected pcDNA plasmid in 293T cells. After 3 days, I harvested the cell and extracted the whole protein. Then, I performed WB to test transfection...

15 January 2021 8,312 0 View

Similar questions and discussions
Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...

01 August 2024 9,993 3 View

For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

31 July 2024 5,113 4 View

What are the factors that causes the sample to have higher efficacy at lower concentration?

We conducted an antibacterial study of a plant extract. Varying concentrations of crude extract were subjected to microbroth dilution assay. The result showed that only the lowest concentration of...

31 July 2024 8,666 3 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

How can I extract my bibliography from researchgate ?

how can I extract my bibliography from researchgate ?

28 July 2024 6,737 1 View

How can biogenic synthesis techniques be applied to develop plants capable of extracting and processing heavy metals from contaminated soil, and wha?

Understand how to utilize biosynthesis to develop natural and sustainable solutions to address heavy metal pollution and improve environmental and agricultural conditions.

26 July 2024 1,469 1 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close