Goodevening all,
Thanks for thy help few weeks ago regardiing lactic acid bacteria.
i did IL-1beta and IL-8 ELISA experiemnt, i could see that Control's yellowish color was as strong as LPS treated group.(which should not...)
I cultured human gingival epithelial cell (YD-38 cell line isolated from human lower gingiva)
in 96 well plate until 70~80% confluent. stimulated it with P. gingivalis LPS (1microgram/ mL) for 24 hr. then collected its supernatant respectivly.
i measured IL-1beta and IL-8 pg/mL using ELISA kit.
and IL-8 pg/mL of control was as same as that of P.gingivalis LPS stimulated.
This result was same in IL-1beta ELISA.
i looked in to papers of YD-38 but i saw that control gorup had significantly lower IL-8 and IL1beta secretion. but mine dont.
I know that most of unstimulated cells have endogenous IL-1beta and very few IL-8 secretion
however, those should be not much as the ones treated with LPS....
what could be the solution for this...? here are the pictures.
Thanks alot and god bless
you should confirm that IL-1 beta is too high in control group or too low in LPS-treated group.
Hi Zhou, I tried 10 fold dilution on samples. and it did work. i can see the statistically significant differences between all samples!
hi guys try to increase the amount of LPS in to 2 5 10 microgram /mL and it worked!
my NT and LPS groups are now significantly different!
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