Donghan Kim

6 Questions 17 Answers 0 Followers

Questions related from Donghan Kim

RAW-OC, optimum incubation time for NFATc1, and cfos fold increase?

Goodevening! I am currently optimizing stimulation time for RANKL induced RAW-Osteoclast for qPCR analysis. for osteclast markers such as TRAP, MMP-9, Cathepsin K, a significant mRNA fold...

20 May 2022 8,012 1 View

IL 1beta secretion in RAW 264.7CELLS?

Hi Sir and Ma'am. I rescently isolated a sample from probiotic strain and found that it suppress LPS induced TNF Alpha and IL-6 mRNA and proteuin secretion in RAW 264.7 cell. But here is...

15 November 2021 2,142 0 View

Aspirating MTT Formazan crystals drying well?

Hi guys. Im rescently having trouble with my MTT assay using RAW 264.7 CELLS. that when ever i aspirate MTTreagent and spent media before adding DMSO, my crystals gets rid off from the bottom of...

11 October 2021 3,756 5 View

I gram-stained SINGLE COLONY from Lactobacillus brevis stock steaked on MRS and found gram positive and negative bacilli?

Hi there, i rescently opened a 3 new stocks of Lactobacillus brevis and streaked them in a seperate MRS Agar. after two days of incubation at 37 degree celcius i found single colonies and gram...

17 August 2021 7,218 3 View

Why there is no difference between Control and P.gingivalis LPS stimulated sample in IL-1beta and IL-8 expression level in gingival epithelial cell?

Goodevening all, Thanks for thy help few weeks ago regardiing lactic acid bacteria. i did IL-1beta and IL-8 ELISA experiemnt, i could see that Control's yellowish color was as strong as LPS...

24 May 2021 3,536 3 View

How can I get growth of Lactic Acid Bacteria in MRS broth?

Im currently working on growing LAB strains isolated from Kefir drink... those LAB stocks grew faily good on MRS Agar plate. (incubated for 24hr at 37'C) so, i took loopful of colonies and...

18 April 2021 8,977 8 View