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Questions related from Donghan Kim
Goodevening! I am currently optimizing stimulation time for RANKL induced RAW-Osteoclast for qPCR analysis. for osteclast markers such as TRAP, MMP-9, Cathepsin K, a significant mRNA fold...
20 May 2022 7,974 1 View
Hi guys. Im rescently having trouble with my MTT assay using RAW 264.7 CELLS. that when ever i aspirate MTTreagent and spent media before adding DMSO, my crystals gets rid off from the bottom of...
11 October 2021 3,716 5 View
Hi there, i rescently opened a 3 new stocks of Lactobacillus brevis and streaked them in a seperate MRS Agar. after two days of incubation at 37 degree celcius i found single colonies and gram...
17 August 2021 7,174 3 View
Goodevening all, Thanks for thy help few weeks ago regardiing lactic acid bacteria. i did IL-1beta and IL-8 ELISA experiemnt, i could see that Control's yellowish color was as strong as LPS...
24 May 2021 3,496 3 View
Im currently working on growing LAB strains isolated from Kefir drink... those LAB stocks grew faily good on MRS Agar plate. (incubated for 24hr at 37'C) so, i took loopful of colonies and...
18 April 2021 8,938 8 View