Why there is age restriction for Rota virus vaccination?

More Lubna Jamal Abdulmalek's questions See All

What is the acceptable limit of radon gas and its radiological effects in fresh canned meat ?

One of the most crucial elements of the human diet, according to experts, is meat products. The world's output of meat products is rising, which is related to both rising levels of consumption in...

19 March 2024 7,310 5 View

Is it possible to conduct a mini ethnography case study with just an in-depth interview?

HRM

11 December 2023 7,176 2 View

In a cell viability assay Is it acceptable to adjust results according to seeded cells number?

Hi Is it acceptable to adjust results (RFU) cell viability assay (ATP), According to number of cells seeded, I have done three replicates of an experiment were the number of cells I have seeded in...

24 October 2023 9,737 1 View

What software do you use to manage your research project?

What tools/softwares/apps you use to track milestones & monitor the workflow of your research?

16 October 2023 9,154 3 View

Correlation of XRD &TEM results of nanoparticles with the help of Springe pattern & SAED pattern?

Can anyone help me in explaining the relationship between XRD &TEM data obtained for nanoparticles with the help of Springe pattern &SAED pattern?

14 August 2023 6,495 3 View

How to eliminate ghost band in Urea polyacrylamide gel ?

I performed in vitro DNA replication. and run the product on 15 % urea polyacrylamide gel after 5min denaturation in 95 oC. I have been doing it for several times and works fine, however, recently...

08 July 2023 3,410 0 View

Samples clustering by time by machine learning techniques, how to assess clusters’ sample similarity by time?

I have a longitudinal dataset for 500 sensors reading at 25 time point. I want to cluster those sensor readings at each time point , from T1 to T25 bur I want to know which cluster each sensor...

27 June 2023 4,571 4 View

Hi, I am willing to seed CaCo2 cells on 24 wells transwell - need a help with how to seed(how much) and more info. about the differntiation process?

how "much" cells I need to seed in a transwell and how to assure getting a monolayer?

09 March 2023 3,269 0 View

How to get rid of the host proteins (E.coli) during my protein purification ?

am trying to purify a human DNA binding protein (normally localized in nucleus and mitochondria). I am currently optimizing the conditions I tried 2 different approaches: 1. HisTrap-> TEV -->...

05 December 2022 5,053 5 View

What are the existed method to visualize longitudinal datasets?

I want to visualizing the temporal relationship of patients records over time, assuming that i have EHR dataset, what are the used techniques to plot or visualize such kind of dataset, where...

22 November 2022 8,899 3 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Can I detect the FLAG sequence of my AAV vector by qPCR?

I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...

14 July 2024 8,482 3 View

Intracerebroventricular (ICV) AAV inj.?

I'd like to know if anyone has got experience in intracerebroventricular (ICV) AAV inj. I am looking for a concomitant spread of the AAV including but not limited to the motor cortex and the...

12 July 2024 781 0 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,669 5 View

Does anyone know how to improve calcium-imaging fluorescence with GRIN lenses?

Hey everyone, I've been working with GRIN lenses (Inscopix) for almost 1 year and still had no luck seeing strong fluorescence on them. When I look at them, I can see good blood flow and,...

08 July 2024 5,178 2 View

Why do I have two different bands from the same virus after PCR?

I get two different bands from BCMV after PCR

07 July 2024 2,112 1 View

Problems to find a region of a Virus gene?

We have a couple of primers but when we make a blast for those primers we see two results: When I use Blast from NCBI, I found many lineages which anniling with many lineages. But when I want to...

01 July 2024 7,644 3 View

I need this article, can anyone get it?

I need this complete article, could anyone help me? follow theme below. Chikungunya virus cell-to-cell transmission is mediated by intercellular extensions in vitro and in vivo.

20 June 2024 3,373 1 View

Can anybody recognize the virus on potato plants?

Infected potato plant was negative for PVY, PVX, PVM, PVS. What can it be?

19 June 2024 6,739 3 View

Protocol for Antibody Dissociation from Virus in Serum Samples?

Hello everyone, I'm currently working on a project where I need to isolate viruses in culture from incubation with PCR-positive serum samples, but I'm facing the challenge of dissociating...

17 June 2024 1,855 1 View