how "much" cells I need to seed in a transwell and how to assure getting a monolayer?
One of the most crucial elements of the human diet, according to experts, is meat products. The world's output of meat products is rising, which is related to both rising levels of consumption in...
19 March 2024 7,310 5 View
HRM
11 December 2023 7,176 2 View
Hi Is it acceptable to adjust results (RFU) cell viability assay (ATP), According to number of cells seeded, I have done three replicates of an experiment were the number of cells I have seeded in...
24 October 2023 9,737 1 View
What tools/softwares/apps you use to track milestones & monitor the workflow of your research?
16 October 2023 9,154 3 View
Can anyone help me in explaining the relationship between XRD &TEM data obtained for nanoparticles with the help of Springe pattern &SAED pattern?
14 August 2023 6,495 3 View
I performed in vitro DNA replication. and run the product on 15 % urea polyacrylamide gel after 5min denaturation in 95 oC. I have been doing it for several times and works fine, however, recently...
08 July 2023 3,410 0 View
I have a longitudinal dataset for 500 sensors reading at 25 time point. I want to cluster those sensor readings at each time point , from T1 to T25 bur I want to know which cluster each sensor...
27 June 2023 4,571 4 View
am trying to purify a human DNA binding protein (normally localized in nucleus and mitochondria). I am currently optimizing the conditions I tried 2 different approaches: 1. HisTrap-> TEV -->...
05 December 2022 5,053 5 View
I want to visualizing the temporal relationship of patients records over time, assuming that i have EHR dataset, what are the used techniques to plot or visualize such kind of dataset, where...
22 November 2022 8,899 3 View
I want to learn and include the temporal relationship alongside my variables at each time series instance. And I learnt that previously they used models to represent all variables by a single...
02 November 2022 5,433 1 View
Does matter flow through ecosystems and energy is recycled and how does the flow of matter and energy compare in the living world?
03 August 2024 607 2 View
I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...
02 August 2024 1,178 3 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...
02 August 2024 8,572 0 View
How do living organisms play a role in the water cycle and why is nonpoint source pollution potentially more harmful than point source pollution?
01 August 2024 7,061 2 View
As the construction industry advances, the integration of Project Data Analytics and Artificial Intelligence (AI) is becoming increasingly crucial in project management. These technologies are...
31 July 2024 6,484 1 View
I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...
30 July 2024 2,301 7 View
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25 July 2024 6,938 3 View
Hello all, Recently as part of a research project at university, we have been studying the effects of light and temperature on eucalyptus seed germination. In our initial run of trials we...
24 July 2024 8,850 4 View
Kao's panel cointegration tests , is there anyone willing to explain me the eviews-9 output for the Kao's panel cointegration tests?
23 July 2024 5,051 4 View
Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...
20 July 2024 4,143 2 View