In general DNA targeted chemotherapeutic drug, interact with DNA and inhibit the DNA function finally cause the cell death (both normal and cancer cell). To understand the DNA binding ability of the drug DNA binding studies have been carried out.
But, In PDT, photosensitizer kill the cells in the presence of light. Then what is the meaning to study DNA binding affinity of the photosensitizer. Can't it inhibit the DNA function and kill the cell as normal DNA targeted chemotherapeutic drug?.
ok if the photosensitizer shows weak interaction with DNA then it won't kill the cell via DNA inhibition the cell death only cause by ROS generation. but many papers reported that photosensitisers show more intercalation interaction with DNA and said that ROS generation is main cause for Cancer cell death. Why not DNA binding affinity is not the reason for cell death.
Then what is the use for DNA binding studies? Is it just add more data to the article?
PS-DNA interaction has been studied due to PS toxicity (at high concentration) under dark conditions.
In PDT (in vivo) TPPS and TMPyP porhyrins have been relocalized into the nucleus after irradiation. Then interaction with DNA may not always lead to cell death, it may induce mutations.
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