Any idea on how to make an intractive chromosome Ideograms?

I am trying to make a chromosome ideogram (map genes on a chromosome) in an interactive way; if you click on a section of the chromosome, it expands and shows the corresponding genes in that region.

27 January 2019 9,858 3 View

How to mitigate the gap between the concept and implementation of the Integrated water resource management ??

Working on the sustainability of the water basins, I focused more on the current approach of Integrated Water Resource Management. There are two main challenges in the real projects: 1: The...

14 October 2018 5,634 10 View

How to convert a UMAT into VUMAT?

28 June 2017 4,714 1 View

Are k sparing diuretics (tiramterine and amiloride) filtered or secreted to reach ENaC?

How do triamterene and amiloride reach their target in the collecting duct lumen?

11 January 2017 4,385 1 View

Viscoelastic Material definition in Abaqus ?

dear colleaguesIn the picture you can obsrve the Stress-Strain hysteresis loop for two different materials. I need to know how can I use these cyclic test data to define viscoelasic behavior in...

13 September 2016 5,406 2 View

Error using iwgdx in matlab for Gams and matlab interface

Hello. Can anybody help me about using iwgdx inorder to linking matlab and Gams. matlab give me the error : 'Undefined function for input argument of type 'char' '. I can use wgdx and rgdx but...

05 July 2016 4,350 4 View

How is Symmetry definition in abaqus for this case ?

In the Picture below you can see the geometry which I should investigate.The one in the left is the complete geometry , and the one in the right is the one which I should use as the model in...

14 March 2016 8,488 3 View

How to define symmetry with respect to 2 Coordinate system in Abaqus?

Dear Colleagues I need to define some symmetry condition for my model in Abaqus and I have to use at least two Coordinate systems.But Each Time I run the analysis it gives me an error which tells...

13 March 2016 6,050 3 View

Why is there no MFI in my LUMINEX experiment?

recently I run a Portca assay 20 plex and I face a problem. The problem that I face is the MFI for my samples are very low (less than Zero). For standards the few first high dilution the MFI are...

09 January 2015 9,504 3 View

Is it possible to plot time solution of variables against time in AUTO 07P, such that the time axis is not limited to the value of 1?

Hello all, I am using Bifurcation and Continuation Software AUTO-07p, for my thesis. I need to plot some of the variables of my system, against time. However, solutions are only plotted for the...

27 December 2014 4,317 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View

Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

How do I fix tissue rolling while cutting frozen rat brain section with a cryostat?

I am cutting rat brain sections at 20um at -20C. The tissue is cutting nicely out of the O.C.T solution, but I can not get a decent section to adhere to the slide. I have included an image.

09 February 2021 1,345 5 View

Rat Macrophage Markers for Flow Cytometry?

Hello, I'm trying to identify good putative macrophage markers for flow cytometry in rats.

04 February 2021 3,913 4 View

Does anyone know how to isolation of high-purity myenteric plexus from adult rat gastrointestinal tract for further biochemical investigation?

I would like to isolate myenteric plexus from the rats small intestine and then isolate certains components of ENS (enteric nervous system) by biochemical tests.

04 February 2021 6,068 2 View

How to do rat bone Immunofluorescence, Please suggest?

Hello, I have been trying to do a rat bone Immunofluorescence histology study. But not able to find a successful protocol till now, Any suggestions? Rat bone: Decalcifies Embedding: Cryo

26 January 2021 4,165 3 View

The litter effect and what can I do to avoid it?

My thesis involves experimental study with Wistar puppies. I am dividing the puppies equally in relation to the groups. However I only have 4 mothers and I will use all of their puppies. There is...

25 January 2021 6,965 3 View