How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How can Gamma Ray API units be converted to CPS (counts per second)?

Oil and gas companies run gamma ray tool and take readings in API units while Gamma ray is also noted in cps, so how these units can be converted into each other?

24 February 2021 7,545 2 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View

XRPD Quantification - Building a calibration curve of drug excipient?

I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...

10 February 2021 6,145 3 View

Student writing his own Learning Outcomes - is this the way forward?

'Students just dont want to study; they are here to waste time!' I have heard this phrase many times; so are the students solely to be blamed? We will find many students who just dont seem to...

04 February 2021 8,546 22 View

What's the difference between light absorption and coupling in plasmonic metals?

What's the difference between light absorption and coupling in plasmonic metals? It seems that light absorbed in metal nanostructure/nanoparticles doesn't contribute to enhancement in electric...

14 January 2021 1,884 4 View

How to increase ductility of bitumen?

What's the optimum amount of sulfur or polyphosphoric acid that could add to bitumen for increase ductility without negative influence on viscosity and other parameters?

11 January 2021 985 4 View

Why the presence of surface active compound repress the mass spectra of other compound? If the sample is itself surface active, then self repression?

I am assuming the resulting insertion/agglomeration in the presence of a surfactant or the agglomeration of surfactant may reduce the rate of ionization. The mobility of the ion may also be...

07 January 2021 987 5 View

Does the initial cell number play a role in affecting the output variables like IC50, EC50, etc.in cell-based drug screening assays? If so, how?

While performing cell-based assays to assess drug toxicity or drug efficacy, how is the initial cell seeding density determined? What is the standard followed in the pharmaceutical industry while...

07 January 2021 6,983 3 View

Is it necessary to validate the control test for each FFP separately?

Hi everyone, I would like to ask if it is necessary to validate the control test (eg sub-visible particles) for each Finished Pharmaceutical Product (FPP) separately (using the same method, the...

06 January 2021 5,568 3 View

Does anyone know about vision-guided control of civil structures?

I would like to know more about the following issues: 1. Using images as feedback for the closed-loop control of civil structures 2. Simulating the vision-guided control on the computer without...

03 January 2021 7,566 1 View