27 August 2020 2 476 Report

I perform glucose isomerization to fructose and separate the two components in hplc ROA organic acid column. I prepared calibration curves for fructose and glucose using signal of refractive index (RI) detector to calculate corresponding concentration. However, I got reaction selettivity larger than 1 (selectivity=desired fructose/ consumed glucose). Is it because the measurement of HPLC didn't give accurate result? Thank you!

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