What is the observed molecular weight of Immediate early gene Egr-1 in mouse cortex whole cell lysates?

Hi everyone, I used anti-Egr-1 (rabbit monoclonal, ab133695, Abcam) in western blotting experiment and I see multiple bands ranging from 50kDa to 130kDa, where at 130kDa bands are more intense...

02 March 2020 3,058 1 View

Why Tau 46 is considered as a biomarker for taupathies?

Hi, Tau 46 antibody is used to tag total tau. What does total tau mean? Tau isoforms + phosphorylated tau? In IHC, I observe positive staining in transgenic mice (Tg4510) hippocampus, and in...

11 December 2018 4,959 0 View

What is the difference between microRNA and siRNA ?

Hi everyone, Even though the definitions and descriptions are available online about microRNA ans siRNA, I want to get an insight through the scientists working on it and could give me a...

06 July 2018 5,325 1 View

If anyone can suggest me the source of caspase-8 antibody best working for mouse brain tissue?

Hi, I have to do IHC staining of caspase-8 in AD tg mouse brain tissue. If anyone can provide the best source and protocol for IHC-P staining of mouse brain tissue with caspase-8, it will be...

04 May 2018 6,038 1 View

Can anyone provide the protocol for IHC-P for the immunostaining with Cleaved Caspase-3 (Asp175) Antibody #9661?

With reference to the question, if anyone can share the reference paper or protocol for IHC- P staining and depicting the staining pattern in brain tissue for cleaved caspase 3. Thanks,

04 May 2018 7,857 0 View

Does immunostaining of total caspase 3 indicate the activation of caspase pathway for apoptosis?

31 December 2017 4,724 3 View

Does unilateral entorhinal cortex lesion lead to changes in contralateral hippocampi??

I came across some literature showing the evidences of equally altered gene expression of some markers in contralateral site in stroke and EC Lesion. In my experiment, from imuunohistochemistry...

11 December 2017 3,919 1 View

Can anyone provide the protocol for Acridine orange/Ethidium bromide staining to visualize apoptosis?

Hi everyone, Here I am working on neurodegeneration model and I am in a need to visualize apoptosis in early as well as late phase. I am aware of TUNEL assay, but I am more interested in...

08 September 2017 2,146 4 View

How to use cell counting plugin in Image J?

I need to count nerve cells in the tissue stained with Cresyl violet. Please provide me guide to make us of Image J software for cell counting. I have cell counter plugin in Image, but I don't...

07 August 2017 3,143 1 View

If I am using microwave oven for antigen retrieval step in IHC, what could be the ideal exposure time?

Hi, Before I used to use waterbath for antigen retrieval step (citrate buffer) for IHC, but now I am advised to use microwave. I performed IHC 2-3 times, but I observe partial staining or no...

04 May 2017 6,901 10 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Should we reduce the standard unit of length to 0.31951540592 meter ?

The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...

27 February 2021 947 7 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

Methodological confusions regarding deductive and inductive reasoning, hypothesis and theory test?

Hi Colleagues- What do you think about the following methodological issues: 1. Is it methodologically wrong to formulate quantitative research questionnaire based on inductive reasoning? 2. Is it...

21 February 2021 4,634 6 View