Can anyone provide the protocol for IHC-P for the immunostaining with Cleaved Caspase-3 (Asp175) Antibody #9661?

05 May 2018 0 8K Report

With reference to the question, if anyone can share the reference paper or protocol for IHC- P staining and depicting the staining pattern in brain tissue for cleaved caspase 3.

Thanks,

Badges
Science topic

More Amisha Parmar's questions See All

What is the observed molecular weight of Immediate early gene Egr-1 in mouse cortex whole cell lysates?

Hi everyone, I used anti-Egr-1 (rabbit monoclonal, ab133695, Abcam) in western blotting experiment and I see multiple bands ranging from 50kDa to 130kDa, where at 130kDa bands are more intense...

02 March 2020 3,184 1 View

Why Tau 46 is considered as a biomarker for taupathies?

Hi, Tau 46 antibody is used to tag total tau. What does total tau mean? Tau isoforms + phosphorylated tau? In IHC, I observe positive staining in transgenic mice (Tg4510) hippocampus, and in...

11 December 2018 5,057 0 View

What is the difference between microRNA and siRNA ?

Hi everyone, Even though the definitions and descriptions are available online about microRNA ans siRNA, I want to get an insight through the scientists working on it and could give me a...

06 July 2018 5,426 1 View

If anyone can suggest me the source of caspase-8 antibody best working for mouse brain tissue?

Hi, I have to do IHC staining of caspase-8 in AD tg mouse brain tissue. If anyone can provide the best source and protocol for IHC-P staining of mouse brain tissue with caspase-8, it will be...

04 May 2018 6,124 1 View

Does immunostaining of total caspase 3 indicate the activation of caspase pathway for apoptosis?

Hi everyone! I did immunostaining of sham and stroke tissue with Total caspase 3 ( Caspase-3 Antibody #9662), and I could able to see staining in sham animal tissue too. So, is it normal to see...

31 December 2017 4,822 3 View

Does unilateral entorhinal cortex lesion lead to changes in contralateral hippocampi??

I came across some literature showing the evidences of equally altered gene expression of some markers in contralateral site in stroke and EC Lesion. In my experiment, from imuunohistochemistry...

11 December 2017 4,021 1 View

Can anyone provide the protocol for Acridine orange/Ethidium bromide staining to visualize apoptosis?

Hi everyone, Here I am working on neurodegeneration model and I am in a need to visualize apoptosis in early as well as late phase. I am aware of TUNEL assay, but I am more interested in...

08 September 2017 2,254 4 View

How to use cell counting plugin in Image J?

I need to count nerve cells in the tissue stained with Cresyl violet. Please provide me guide to make us of Image J software for cell counting. I have cell counter plugin in Image, but I don't...

07 August 2017 3,234 1 View

Why protein blocking step is needed before the application of primary antibody in IHC?

I am curious to know the possible reasons of protein blocking step and how does it affect the final result if we skip the step. Thanks in advance!

07 August 2017 4,821 0 View

If I am using microwave oven for antigen retrieval step in IHC, what could be the ideal exposure time?

Hi, Before I used to use waterbath for antigen retrieval step (citrate buffer) for IHC, but now I am advised to use microwave. I performed IHC 2-3 times, but I observe partial staining or no...

04 May 2017 7,102 10 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,282 1 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View