Hi,
I have to do IHC staining of caspase-8 in AD tg mouse brain tissue. If anyone can provide the best source and protocol for IHC-P staining of mouse brain tissue with caspase-8, it will be helpful.
Thanks,
Article Caspase Protocols in Mice
Hi everyone, I used anti-Egr-1 (rabbit monoclonal, ab133695, Abcam) in western blotting experiment and I see multiple bands ranging from 50kDa to 130kDa, where at 130kDa bands are more intense...
02 March 2020 3,184 1 View
Hi, Tau 46 antibody is used to tag total tau. What does total tau mean? Tau isoforms + phosphorylated tau? In IHC, I observe positive staining in transgenic mice (Tg4510) hippocampus, and in...
11 December 2018 5,057 0 View
Hi everyone, Even though the definitions and descriptions are available online about microRNA ans siRNA, I want to get an insight through the scientists working on it and could give me a...
06 July 2018 5,426 1 View
With reference to the question, if anyone can share the reference paper or protocol for IHC- P staining and depicting the staining pattern in brain tissue for cleaved caspase 3. Thanks,
04 May 2018 7,956 0 View
Hi everyone! I did immunostaining of sham and stroke tissue with Total caspase 3 ( Caspase-3 Antibody #9662), and I could able to see staining in sham animal tissue too. So, is it normal to see...
31 December 2017 4,822 3 View
I came across some literature showing the evidences of equally altered gene expression of some markers in contralateral site in stroke and EC Lesion. In my experiment, from imuunohistochemistry...
11 December 2017 4,021 1 View
Hi everyone, Here I am working on neurodegeneration model and I am in a need to visualize apoptosis in early as well as late phase. I am aware of TUNEL assay, but I am more interested in...
08 September 2017 2,254 4 View
I need to count nerve cells in the tissue stained with Cresyl violet. Please provide me guide to make us of Image J software for cell counting. I have cell counter plugin in Image, but I don't...
07 August 2017 3,234 1 View
I am curious to know the possible reasons of protein blocking step and how does it affect the final result if we skip the step. Thanks in advance!
07 August 2017 4,821 0 View
Hi, Before I used to use waterbath for antigen retrieval step (citrate buffer) for IHC, but now I am advised to use microwave. I performed IHC 2-3 times, but I observe partial staining or no...
04 May 2017 7,102 10 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View