Amisha Parmar

12 Questions 3 Answers 0 Followers

Questions related from Amisha Parmar

What is the observed molecular weight of Immediate early gene Egr-1 in mouse cortex whole cell lysates?

Hi everyone, I used anti-Egr-1 (rabbit monoclonal, ab133695, Abcam) in western blotting experiment and I see multiple bands ranging from 50kDa to 130kDa, where at 130kDa bands are more intense...

03 March 2020 3,140 1 View

Why Tau 46 is considered as a biomarker for taupathies?

Hi, Tau 46 antibody is used to tag total tau. What does total tau mean? Tau isoforms + phosphorylated tau? In IHC, I observe positive staining in transgenic mice (Tg4510) hippocampus, and in...

12 December 2018 5,023 0 View

What is the difference between microRNA and siRNA ?

Hi everyone, Even though the definitions and descriptions are available online about microRNA ans siRNA, I want to get an insight through the scientists working on it and could give me a...

07 July 2018 5,390 1 View

Can anyone provide the protocol for IHC-P for the immunostaining with Cleaved Caspase-3 (Asp175) Antibody #9661?

With reference to the question, if anyone can share the reference paper or protocol for IHC- P staining and depicting the staining pattern in brain tissue for cleaved caspase 3. Thanks,

05 May 2018 7,919 0 View

If anyone can suggest me the source of caspase-8 antibody best working for mouse brain tissue?

Hi, I have to do IHC staining of caspase-8 in AD tg mouse brain tissue. If anyone can provide the best source and protocol for IHC-P staining of mouse brain tissue with caspase-8, it will be...

05 May 2018 6,098 1 View

Does immunostaining of total caspase 3 indicate the activation of caspase pathway for apoptosis?

Hi everyone! I did immunostaining of sham and stroke tissue with Total caspase 3 ( Caspase-3 Antibody #9662), and I could able to see staining in sham animal tissue too. So, is it normal to see...

01 January 2018 4,786 3 View

Does unilateral entorhinal cortex lesion lead to changes in contralateral hippocampi??

I came across some literature showing the evidences of equally altered gene expression of some markers in contralateral site in stroke and EC Lesion. In my experiment, from imuunohistochemistry...

12 December 2017 3,984 1 View

Can anyone provide the protocol for Acridine orange/Ethidium bromide staining to visualize apoptosis?

Hi everyone, Here I am working on neurodegeneration model and I am in a need to visualize apoptosis in early as well as late phase. I am aware of TUNEL assay, but I am more interested in...

09 September 2017 2,217 4 View

How to use cell counting plugin in Image J?

I need to count nerve cells in the tissue stained with Cresyl violet. Please provide me guide to make us of Image J software for cell counting. I have cell counter plugin in Image, but I don't...

08 August 2017 3,200 1 View

Why protein blocking step is needed before the application of primary antibody in IHC?

I am curious to know the possible reasons of protein blocking step and how does it affect the final result if we skip the step. Thanks in advance!

08 August 2017 4,791 0 View

If I am using microwave oven for antigen retrieval step in IHC, what could be the ideal exposure time?

Hi, Before I used to use waterbath for antigen retrieval step (citrate buffer) for IHC, but now I am advised to use microwave. I performed IHC 2-3 times, but I observe partial staining or no...

05 May 2017 7,050 10 View

Can anyone explain any experimental method that can be used to isolate CA1 rich region from the whole hippocampus?

Hi everyone, I need to isolate CA1 rich region from the whole hippocampus, I am aware about the advanced technique (by the use of vibratome) used to isolate CA1 using vibrotome, but I want to...

04 April 2017 1,178 2 View