What are your alternative data collection methods during the pandemic? What are the pros and cons?

Dear colleagues We are exploring possible data collection methods for environment and behavior research in the context of architectural environments. Have you conducted any Post Occupancy...

16 February 2021 2,575 4 View

Quantification of GC-MS data, issue with Masshunter, Any ideas?

Hi, I am using the Masshunter software to quantify my GC-MS data. So, did the integration using MassHunter. but the data (area) are weird. I have a lot of peaks in one chromatogram, therefore,...

09 November 2020 2,181 5 View

How to apply cyclic loading to a discrete model of a masonry wall in 3DEC software?

It is possible to apply a monotonic load to a URM wall in 3DEC code but I am wondering is it possible to apply a cyclic load there like FEM softwares?

10 February 2020 3,237 3 View

Can pET expression vectors get used for sequencing and storage?

I was wondering if we could skip one step in cloning process from sequencing vector to expression vector? Meaning that start cloning the gene directly into expression vector such as pET 28b and do...

04 February 2020 9,742 2 View

Do you recommend ligation free cloning system?

I plan to clone some terpene synthase genes into a sequencing vector and later do the heterologous expression using an expression vector. I was wondering if anyone has experience with free...

28 January 2020 7,751 3 View

Why R studio deletes some rows from the heatmap/ doesn't show all genes?

I am using Rstudio to create heatmap for some expression data; My file includes 38 genes, therefore, 38 rows and 20 columns as samples. The output heatmap shows only 19 of my genes!!! Any idea how...

10 December 2019 7,530 3 View

What software/program you recommend to convert fasta file to Nexus.nxs?

I need to convert my fasta format alignment file to "Nexus.nxs" to be able to use it in Mybeys. What is your suggestion? So far the files I created using Mega x as well as an online database...

09 October 2019 4,362 2 View

Problem with trimAL installation?

Is anyone can help in how to install trimAL on windows 10? I am gonna use the software for trimming of my aligned sequences. It seems the software does not get installed in my system! Any idea...

15 September 2019 6,068 3 View

What will be the programming language of future Computational Codes? Good old FORTRAN evolves or will be removed from heavy duty computational world?

I know there are great reliable libraries on Fortran for Computational PDE/ODE, Computational Statistics and Numerical Linear Algebra. Also almost all the legacy software packages of Civil...

30 August 2018 5,424 4 View

Do I need to design a specific antibody for purification of my small recombinant peptides?

I am fusing small peptides around 10 to 30 a.a into the C-terminus of a larger protein. The question is if I need to design specific antibodies against those small epitopes? I actually have two...

10 May 2018 1,091 1 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Will automated killing diminish the error rate by police?

10 December 2020 5,548 3 View

Where can I find a database of antibiotics-dose required to kill bacteria?

Hi, I am Zaizhi. I would like to know where I can find a database of antibiotics-dose required to kill different bacteria. I found a few years ago. There, I was able to know the range of dose of...

08 November 2020 9,276 3 View

How can i collect many blood sample from the rat for pharmacokinetic study without killing the rat??

how can i collect many blood sample from the rat for pharmacokinetic study without killing the rat??

28 October 2020 2,675 8 View

What statistical analysis method should I use with multivariate categorical data?

Hi, I'm struggling quite a bit, Hope someone can help. My research question is roughly to what extent (and what) characteristics of murder and manslaughter cases are related. The data consists...

05 October 2020 3,958 8 View

If the microalgae experience stress through transportation. What would be the best course of action to avoid it from becoming contaminated or killed?

I am currently doing an experiment in my faculty lab and I need living micro-algae to be transported from other countries. The samples will be done in Morocco and Spain and they will be sent to...

08 September 2020 4,424 2 View

What are Analgesics or Painkillers ? Do you think that Painkillers really kill the pain or it is a misleading word ?

Does the pain really goes away or it is something else ??

08 September 2020 133 19 View

How do people usually measure bacterial interactions?

How do people usually measure bacterial interations? Thank you!

20 August 2020 7,472 3 View

Acidic hydrogel kills cells?

As hydrogel was prepared in acidic medium, released acid from hydrogel kills cells in in-vitro test. I am wondering to compromise such issue.

12 August 2020 9,319 3 View

HEK-293t cell detach but still alive during killing curve set up?

Hi there, I'm trying to build a killing curve for HEK-293T towards hygromycin B for the following transfection experiment. I plated the cell at 1.3X10^5 cell/well in the 24 well plates the night...

30 June 2020 7,368 1 View