There are also other PEGs used in drought stress for example PEG4000 (http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0132314), PEG 8000 (http://onlinelibrary.wiley.com/doi/10.1111/nph.12004/full), and PEG10000 (http://link.springer.com/article/10.1023/A:10213771037759), usually with a molecular weight of more than 4000.

The reason for for using PEGs with large molecular weight is that smaller PEGs can diffuse through the cell wall and are even taken up by plant cells and distributed through the plant, which may influence the results.

because PEG can capture more  water molecular  than plant    

 Go To:

http://www.plantstress.com/methods/index.asp 

... and look for "Using PEG for controlling plant water status in experiments"

Polyethylene glycol molecules with a molecular weight

larger than 6,000 (PEG 6000) are inert, non-ionic and cell impermeable.

They are small enough to influence the osmotic

pressure, but large enough unabsorbed by plants. Therefore, they are frequently used

to simulate osmotic stress.

I agree with response of Deke Xing. PEG 8000 is preferred to avoid any sort of absorption by the plant

Most of the  in vitro and in vivo studies with osmotic stress require an inert, non-ionic material which can exert more  negative water potential in  tissue or leaf , for that PEG 6000 is ideal.

Annamalai Nathan

hello every body

want to ask about a proper concentration of PEG that use with bread wheat, and how to prepare it.

thanks to all

Is there anyone work with PEG 6000 (powder form) from Sigma-Aldrich?

Dear Subhash K. Mishra

I think this article can help you

Article Effect of PEG-6000 Imposed drought stress on RNA content, Re...

Polyethylene glycol (PEG) is a high-molecular-weight, non-ionic, and non-plasmolyzing compound that imitates drought stress in cultured cells similar to that observed in the cells of intact plants subjected to drought conditions.

Refer the follow article

In Vitro Assessment of Kurdish Rice Genotypes in Response to PEG-Induced Drought Stress

It is related to the ability of the molecule to change the osmotic potential without interfering with the permeability of the roots.

Subhash K. Mishra

Uses PEG, mannitol and sorbitol And the most used PEG because polyethylene glycol molecules with a molecular weight larger than 6,000 (PEG 6000) are inert, non-ionic and cell impermeable.

Any mechanism for drought stress by using PEG 6000?

How do I calculate the amount of PEG6000 to be added in agar media, to get the desired water potential? Can anybody please help with the calculation?