Why don't need the same number of standard solution and curve as identified from the sample? Why use only one and then compare the peak area of standard with the peak area of each individual compound?
This approach is often used because of practical and financial issues. When we have a complex volatile profile, it is difficult to have all the standards and an external calibration for each one. The values obtained with this strategy are not accurate and can only be used for comparative proposes.
Thank you so much João Micael Leça for your answer. So, you mean using only one standard does not give the exact concentration? But I found all the papers regarding meat volatile used only one standard and then compare the peak area of the standard and the peak area of each specific compound and then quantify. So, their quantification may be tentative.
Exactly is very common in many areas of study. But we can never consider these concentrations as real but comparative. In my case, I already used a single standard to evaluate the behavior of some volatiles and later, when I developed a calibration only with the specific compounds that I wanted to study, the concentration was very different. But the initial results in relation to 1 single internal standard were very useful to define which compounds to follow during my study.
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