I am working in a laboratory with Cov19. Extraction is doing kit but it isn't good and the amount RNA, 260/280 is higher than 3. Can someone help us in this regard?
Hi,
"Abnormal 260/280 ratios usually indicate that a sample is contaminated by residual phenol, guanidine, or other reagent used in the extraction protocol, in which case the ratio is normally low." Do you use Trizol based extraction method?
Hi,
A260/280 ratio greater than 2.2 indicates the presence of basic contamination whereas if ratio decreases 1.9 indicates the presence of acidic phenol or proteins.
Do you have EDTA in your resuspension buffer? I had that problem to and excluding EDTA gave normal values
Check the composition of the last buffer RNA is re-suspended in. It should be written on the second to last page of the kits manual.
What kit is it that you are using? I would also agree with Farid a kit is not necessary especially if you are unsure of the components.
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