Why my protocol for P19 cell differentiation to cardiomyocytes isn't working?

More Neda Karami's questions See All

Do you know any paper about the role of digital content making and translation marketing?

I am determined to write an article in this case and I wonder if anyone can help me in this case. Regards.

25 January 2021 3,330 3 View

How can I fix the floating node error in pspice?

I have a very large circuit and when I try to run a transient analysis of my circuit, there are so many floating node errors, this is the picture of a part of my circuit...

31 December 2020 6,053 5 View

Crop coefficiets (Kc) values more than 1 when estimating ETa using remote sensing?

Hello everyone, I was wondering if anyone knows how to deal with crops with Kc values of more than one when we want to estimate ETa using satellite images for different crops. IS there any...

10 December 2020 9,795 1 View

Why did the authors design a sociodemographic questionnaire?

This is a question from a journal reviewer who asked my manuscript. Can someone please guide me on how to prepare the reviewer's answer? Thanks. here is reviewer question. why did the authors...

16 November 2020 735 2 View

How can I fix the capacity curve of two different structure?

I have two frames for nonlinear analysis, one of them is CBFs and one of them is BRBFs ( the generals are the same, only lateral elements are different). When I drew the capacity curve of my...

11 November 2020 10,034 3 View

How Cryoprotectants works and prevent cell from damage due to freezing?

How these solvents limit shrinking ice crystals during freeze-drying or vitrification? and what are the main differences between DMSO and Glycerol? where each one is useful? I will be grateful if...

17 May 2020 3,540 1 View

The reason for the similarity of RMSF in the protein system and the protein-ligand complex?

The reason for the similarity of RMSF in the protein system and the protein-ligand complex

28 March 2020 3,784 3 View

Why the initial pressure in a fluid cavity simulation in Abaqus fall to zero just at the starting increments?

I am using fluid cavity capability for simulation of a water-filled container with Abaqus-Static-Standard. The container has an initial pressure and is been surrounded by another solid layer with...

21 March 2020 7,153 3 View

Does the presence of clones on the agar plate mean that the cloning has been done successfully?

I did cloning. it was like a traditional approach. Firstly, Restriction digestion was performed for both vector (8kb) and target gen (700bp), and also the vectors and target gene were run on the...

21 March 2020 9,618 4 View

How can I start the simulation of esd effects on satellite solar panels? I'm looking for a start point for esd simulations by softwares likeCST,Hfss?

In all the papers the esd test model consists of a vaccum chamber in which samples exist. There is a plasma simulator or electron gun that simulates the space condition. these induce the esd on...

20 March 2020 5,938 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

Is RD-ES grow attached or in suspension or both?

We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...

24 February 2021 5,183 1 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Neda Karami

Is it because I am using DMEM/F12 instead of DMEM?

Saurabh Mandal

What is the differentiation inducer you have used? DMSO or 5-Aza or TSA treatment is required.

Malcolm Nobre

Hello Neda Karami

Please refer to the article attached below. It may be helpful.

Article Establishment of a model of P19 embryonal carcinoma cells di...

Best.

@saurabh I am using DMSO 1 percent for 4 days