Neda Karami

5 Questions 11 Answers 0 Followers

Questions related from Neda Karami

Why my protocol for P19 cell differentiation to cardiomyocytes isn't working?

I am culturing 1 million cells in 100 mm non adherent bacterial petridishes in DMEM/F12 supplemented with 1% penstrep and 10% FBS for 4 days to form embryoid bodies and then culture them as mono...

19 December 2022 5,153 4 View

Blood brain barrier permeability using FITC-Dextrans?

I am working on emulating a blood brain barrier on a chip and to evaluate paracellular permeability we are using FITC-Dextrans. I am wondering if the permeability of intact blood brain barrier and...

08 February 2022 7,841 1 View

What is the equation for calculating shear stress from volumetric flow rate and geometrical parameters in a rectangular channel?

I want to calculate the shear stress in a rectangular straight/curved channel (the geometry of the rectangle will be fixed), What is the equation that I can use? and also if I want to simulate the...

07 October 2021 1,368 2 View

Determining EC50 : I don't how to graph my data with a 0% control ( no drug but with DMSO 0.05%) that has an absorbance between two of my drug doses?

Hi, I ran an MTT assay with 7 doses of my drug ( dissolved in DMSO , final concentration in media ,0.05%) , and read the absorbance at 490nm according to the reagent manufacturers instructions....

03 September 2018 4,975 3 View

Best protein design software?

what is the best protein design program that can assess the effect of mutation in a globular protein structure? i am looking to change the specificity of a protein. but mostly i am interested to...

31 July 2016 5,347 7 View