Neda Karami

6 Questions 11 Answers 0 Followers

Questions related from Neda Karami

Why my protocol for P19 cell differentiation to cardiomyocytes isn't working?

I am culturing 1 million cells in 100 mm non adherent bacterial petridishes in DMEM/F12 supplemented with 1% penstrep and 10% FBS for 4 days to form embryoid bodies and then culture them as mono...

19 December 2022 5,231 4 View

Blood brain barrier permeability using FITC-Dextrans?

I am working on emulating a blood brain barrier on a chip and to evaluate paracellular permeability we are using FITC-Dextrans. I am wondering if the permeability of intact blood brain barrier and...

08 February 2022 7,915 1 View

What is the equation for calculating shear stress from volumetric flow rate and geometrical parameters in a rectangular channel?

I want to calculate the shear stress in a rectangular straight/curved channel (the geometry of the rectangle will be fixed), What is the equation that I can use? and also if I want to simulate the...

07 October 2021 1,448 2 View

Determining EC50 : I don't how to graph my data with a 0% control ( no drug but with DMSO 0.05%) that has an absorbance between two of my drug doses?

Hi, I ran an MTT assay with 7 doses of my drug ( dissolved in DMSO , final concentration in media ,0.05%) , and read the absorbance at 490nm according to the reagent manufacturers instructions....

03 September 2018 5,089 3 View

Fgf2 and heparin in neural stem cells culture?

to culture E14 mouse cortex Neural Stem Cells, is it efficient to only add EGF to the culture media? or it is a must to add both EGF and FGF2 growth factors? also if adding FGF2, is it necessary...

02 June 2017 2,186 2 View

Best protein design software?

what is the best protein design program that can assess the effect of mutation in a globular protein structure? i am looking to change the specificity of a protein. but mostly i am interested to...

31 July 2016 5,399 7 View