Randy Chance

3 Questions 8 Answers 0 Followers

Questions related from Randy Chance

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

20 February 2021 7,265 5 View

Why my PCR product (band) gets smaller with every round of reamplification?

I use the same primer set and same conditions. The first PCR product from cDNA gave about 800 bp. Reamplification of the template gave 700 bp. A second amplification gave 600 bp. Interestingly, I...

25 February 2020 4,492 8 View

Help with Gibson reaction components calculation please?

An insert length of 811bp (17ng/ul) and digested vector size of 5.6kb (35ng/ul). --100ng vector amount--. Am I right in using the following respective volumes of insert and vector? 2.5ul and...

07 May 2019 7,162 3 View