Hello dear friends
I am working on gene cloning. One of the stages is pCAMBIA 1304 vector extraction.
The low concentration of vector at the final stage of pCAMBIA 1304 vector extraction, is my main problem.
1- How can I solve the problem to increase the concentration?
2- Could anybody recommend any universal primer for the pCAMBIA 1304 vector?
I appreciate your valuable suggestion.
Regards
Akram
Dear Latif ur Rehman
Yes, I had used kit. However, the concentration of vector was very low. Meanwhile, I have not found universal primer for this vector. Do you have any suggestion?
If you are using a kit for plasmid isolation in the kit somewhere it is mentioned the maximum size of plasmid kit can be used and also with % recovery. pCAMBIA1304 is >13 kb plasmid. So please check the size range of the kit you are using.
If you find the kit is not suitable either you can increase the culture volume and proportionally the other reagents for isolation from kit to compensate the loss or Other than this you can try manual protocol for isolation of plasmid DNA.
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