Latif ur Rehman

11 Questions 37 Answers 0 Followers

Questions related from Latif ur Rehman

Anybody using Real Time PCR assay for the genotyping of Fowl Adenoviruses (FAdVs)?

I have used PCR-RFLP for the genotyping of fowl adenoviruses previously but now I am interested in the genotyping of FAdVs by real time PCR. Kindly share your experience if someone has already...

20 May 2021 5,850 3 View

RNase induced degradation of synthetic siRNAs (19nt with 2nt 3' overhang?

Hello guys..Can siRNAs (19 nt with 2 nt 3' overhang) be digested/degraded by RNAses? Although I never met this situation but if anyone has a reference paper in which it is mentioned that it can't...

17 May 2016 2,518 0 View

Expression of a fungal gene under the control of it's own promoter?

I want to express a fungal gene under the control of it's own promoter but there is no reliable online tools to find out where exactly the promoter of that gene is, how many bases upstream or...

04 May 2016 1,581 7 View

How to perform Ferric and cupric reductase assays for Verticillium dahliae?

Anybody have any idea of how to do Ferric reductase and cupric reductase assays for V. dahliae? I need to do these assays but I couldn't found any paper in which they have actually used...

24 March 2016 8,441 0 View

Why do i have smears on gel after restriction digestion with 2 enzymes?

After double digestion of plasmid with Xba1 and HindIII for 4 hours, I run it on gel but unexpectedly each lane was full of smears and the bands were not clearly visible. What can be the possible...

11 March 2016 5,001 10 View

If AGL1 Agrobacterium is transformed with pGKO2 vector without gene insert, can it grow on LB medium with kan antibiotics?

Actually I'm transforming the AGL1 strain with pGKO2 containing our gene construct, but i don't know the reason why the Agrobacterium can't grown after electroporation with the plasmid? what can...

14 September 2015 9,768 5 View

What is the optimum time to see the effect of siRNA on GFP expression in V. dahliae spores?

I am trying to target GFP gene (Spores are already transformed with GFP) by siRNA, after electroporation of the GFP spores with siRNA, what is the optimum time to see the effect on the phenotype?...

21 April 2015 8,002 2 View

How can I control the growth of unwanted spores in the Verticillium dahliae culture?

We are trying to get a pure culture of Verticillium dahliae on PDA, but after sometime green color colonies of some other spores appear on the plate, when seen under microscope, the size is quite...

13 March 2015 414 17 View

How do I increase the permeability of V.dahliae spores?

I want see whether the naked siRNA can enter the V.dahliae spores or not? i mixed fungal spores and siRNA (flourescently labeled) and saw under microscope at various intervals, initially i...

04 February 2015 4,286 5 View

Can we use the CRISPR/Cas9 system for gene deletion in the fungus Verticillium dahliae?

i want to get gene deletion mutant in fungus, as the CRISPR/Cas9 is a rapid method, so i want to apply this method, can anyone tell me about using this method in Fungus?

22 January 2015 9,732 1 View

Is anyone working on Verticillium dahliae?

I have to target some genes of Verticillium dahliae to see their effect on the pathogenicity and development of verticillium dahliae, your suggestions will highly be appreciated. Which vectors...

20 January 2015 9,396 0 View