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Questions related from Latif ur Rehman
Hello guys..Can siRNAs (19 nt with 2 nt 3' overhang) be digested/degraded by RNAses? Although I never met this situation but if anyone has a reference paper in which it is mentioned that it can't...
17 May 2016 2,513 0 View
I want to express a fungal gene under the control of it's own promoter but there is no reliable online tools to find out where exactly the promoter of that gene is, how many bases upstream or...
04 May 2016 1,577 7 View
After double digestion of plasmid with Xba1 and HindIII for 4 hours, I run it on gel but unexpectedly each lane was full of smears and the bands were not clearly visible. What can be the possible...
11 March 2016 4,992 10 View
Actually I'm transforming the AGL1 strain with pGKO2 containing our gene construct, but i don't know the reason why the Agrobacterium can't grown after electroporation with the plasmid? what can...
14 September 2015 9,761 5 View
We are trying to get a pure culture of Verticillium dahliae on PDA, but after sometime green color colonies of some other spores appear on the plate, when seen under microscope, the size is quite...
13 March 2015 410 17 View
I want see whether the naked siRNA can enter the V.dahliae spores or not? i mixed fungal spores and siRNA (flourescently labeled) and saw under microscope at various intervals, initially i...
04 February 2015 4,278 5 View
i want to get gene deletion mutant in fungus, as the CRISPR/Cas9 is a rapid method, so i want to apply this method, can anyone tell me about using this method in Fungus?
22 January 2015 9,726 1 View
I have to target some genes of Verticillium dahliae to see their effect on the pathogenicity and development of verticillium dahliae, your suggestions will highly be appreciated. Which vectors...
20 January 2015 9,391 0 View
