Latif ur Rehman

3 Questions 18 Answers 0 Followers

Questions related from Latif ur Rehman

Why do i have smears on gel after restriction digestion with 2 enzymes?

After double digestion of plasmid with Xba1 and HindIII for 4 hours, I run it on gel but unexpectedly each lane was full of smears and the bands were not clearly visible. What can be the possible...

11 March 2016 4,941 10 View

If AGL1 Agrobacterium is transformed with pGKO2 vector without gene insert, can it grow on LB medium with kan antibiotics?

Actually I'm transforming the AGL1 strain with pGKO2 containing our gene construct, but i don't know the reason why the Agrobacterium can't grown after electroporation with the plasmid? what can...

14 September 2015 9,699 5 View

How can I control the growth of unwanted spores in the Verticillium dahliae culture?

We are trying to get a pure culture of Verticillium dahliae on PDA, but after sometime green color colonies of some other spores appear on the plate, when seen under microscope, the size is quite...

13 March 2015 358 17 View