Aayush Bahl

4 Questions 5 Answers 0 Followers

Questions related from Aayush Bahl

How to isolate RNA from Mycobacterium using Trizol method without the Bead Beater?

I use Lysozyme for cell lysis but I'am not able to isolate RNA from Mycobacterium samples. Please provide me with a detailed protocol.

07 January 2025 8,168 4 View

I'm not able to see the overexpression of my antitoxin on SDS PAGE of my chosen toxin-antitoxin system of mycobacterial species. Is there anything ?

I have been trying to check the overexpression of the antitoxin of my chosen TA system of mycobacterium sp. I have tried to induce the clone at 20, 25 and 37° C at 0.1, 1 and 10 mM concentration...

23 May 2024 1,403 0 View

Why my NiSO4(H2O)6 is not dissolving in 1X PBS buffer solution?

I am trying to recharge the Ni-NTA column for his-tagged protein purification but my NiSO4(H2O)6 is not dissolving in 1x PBS and I have tried it from a different reagent bottle also, please help!

16 May 2024 9,808 3 View

I'm not able to purify my protein, is it due to the column or the protocol?

I'm using Ni-NTA resin (Qiagen) column to purify my his-tagged protein. Over a period of time the color of my column has changed to color less. I'm using the concentration you can see in the...

16 May 2024 2,632 4 View