I am not able to understand some basics. I require a detailed understanding and visual demonstration, as I am going to use it in my next research project.
I need some string insights.
02 July 2024 4,059 1 View
I have been trying to check the overexpression of the antitoxin of my chosen TA system of mycobacterium sp. I have tried to induce the clone at 20, 25 and 37° C at 0.1, 1 and 10 mM concentration...
22 May 2024 1,334 0 View
I'm using Ni-NTA resin (Qiagen) column to purify my his-tagged protein. Over a period of time the color of my column has changed to color less. I'm using the concentration you can see in the...
15 May 2024 2,573 4 View
I am trying to recharge the Ni-NTA column for his-tagged protein purification but my NiSO4(H2O)6 is not dissolving in 1x PBS and I have tried it from a different reagent bottle also, please help!
15 May 2024 9,746 3 View
Or how to create a citation and reference using information available in a paper?
02 May 2024 6,442 5 View
Hi, These measurements are new to me so apologies for the naive questions. How can I use profilometry or ellipsometry to measure the thickness of spin coated quantum dot films on CVD graphene (on...
10 July 2023 1,116 3 View
I have a stack of PbS quantum dots with 1-2 EDT ligands that I need to encapsulate. I have tried encapsulating it with PMMA and SU8 however it seems that the cross linking is being inhibited by...
02 July 2023 7,598 4 View
I have some CVD graphene on copper that I need to cut into circular discs. I can obviously do it using scissors but it wouldn't be super precise and can damage the film in surrounding areas. Would...
20 June 2023 3,543 4 View
We are trying to fabricate free standing thin films (~2um) using SU86002. Since SU86000 is not extensively used in literature, we initially started off with the data sheet parameters and found...
09 April 2023 5,461 1 View
I am trying to find a cloning vector appropriate for fungal transformation. My gene of interest is pgaII from Aspergillus niger. I am planning to insert this gene into a cloning vector and then...
03 May 2022 617 0 View
Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...
07 August 2024 269 3 View
Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...
07 August 2024 7,013 4 View
Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...
24 July 2024 9,337 3 View
Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...
19 July 2024 2,155 0 View
btw have u ever know about math bounded and reality bounded? for example it happen when student interpret the solution of problem toward mathematics. if you have experience or research about that,...
03 July 2024 6,631 2 View
I created Bland Altman Plot to assess the difference, But I want to visualize the difference between the two curves in the same graph.
02 July 2024 5,361 0 View
Specifically, how can these methods integrate various sources of pollution, such as agricultural runoff, industrial discharge, and urban wastewater, to provide a comprehensive and actionable...
27 June 2024 8,562 0 View
Any help will be appreciated!! I am trying to use the Visual MINTEQ to investigate the speciation of ions in some environmental samples. In the input files, I only fixed the pH value and provided...
24 June 2024 9,138 0 View
Hello everyone. I am attempting to do visual cortex imaging in mice anesthetized with halothane, but the survival rate of these mice is quite low, even compared to other mice anesthetized with...
19 June 2024 4,390 4 View
I have seen two kinds of explanations given for the phenomenon that I try to draw in the attached png document. One explanation says that an air bubble creates the experience of a solid black...
16 June 2024 6,493 1 View