I am performing BLI. I have loaded my ligand to activated AR2G sensors via amine coupling. After loading, sensor were quenched with ethanol-amine. Similarly reference sensors were activated and later quenched without loading. But during kinetics my reference sensors are showing more binding than the ligand loaded sensors. I have tried different buffers, 0.1% tween 20, 0.1% SDS and BSA but that didn't help.