Krishna Singh Bisht

4 Questions 7 Answers 0 Followers

Questions related from Krishna Singh Bisht

Why Mice brain cryosections seem porous or web like?

We isolated mice brain after perfusion with chilled PBS, followed by fixation in 4%PFA (24hr) and 30% sucrose dehydration until sank to bottom of tube. Later, brain samples were cryo preserved ....

08 November 2022 7,339 5 View

Why my AR2G reference sensors are showing more binding to analyte in BLI?

I am performing BLI. I have loaded my ligand to activated AR2G sensors via amine coupling. After loading, sensor were quenched with ethanol-amine. Similarly reference sensors were activated and...

22 December 2021 9,411 0 View

Has anyone used CY5.5 reporter probe in taqman assay?

I have a probe labelled with CY5.5 reporter. I want to use this in my taqman assay. But there is no such reporter option available in Applied Biosystem quantstudio 6 system. Can i proceed with...

30 November 2021 604 3 View

Why there is measurement error in my Dynamic Light Scattering Experiment ?

I am performing DLS (Malvern ZetaSizer Nano)experiment for 100uM protein of MW 14.2KDa, pI4.4 dissolved in 20mM Glycine NaOH buffer pH7.4. Samples have been filtered, passed through 100KDa amicon...

19 November 2021 5,106 4 View