why my ABE assay get black?

Jaury Gómez de la Madrid @Jaury-Gomez-De-La-Madrid

11 March 2023 0 9K Report

I use biotin-hpdh 2 uM to biotinylate the protein and i block the membrane with BSA 3% 30 min and streptavidin-HRP 1:10000 , the result is black

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