I have large fluctuations in the individual values in the LDH determination (Kit Promega: CytoTox96 Non radioactive Cytotoxicity Assay) of primary neuron cultures. Anyone know if centrifuging the supernatant would help? Thank you for the answers.
Hi everyone, I'm currently working on a protocol to stain thyroid receptors in zebrafish but I'm having some difficulties with my signal. The protocol I started with included 2% TritonX-100 during...
03 April 2023 1,087 1 View
I'm planning to perform immunohistochemical analysis on adult zebrafish brain slices. Is there are standard for the orientation of the brain slices? I found an article where they analysed 14...
23 November 2022 2,289 0 View
Hello, I am currently conducting research on the speciation analysis of As and other elements using HPLC-ICP MS and ICP-MS. for ICP-MS, I dilute the stock solution of As using 2% HNO3 and make 6...
18 October 2021 7,768 9 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Firing in GABAergic neurons has been characterized in several areas of the brain, but patterns are the same?
04 August 2024 4,090 0 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...
26 July 2024 3,460 2 View
I am starting to work on this question, with particular reference to what we might be able to learn from countries outside the UK. Of course this topic is huge, and I am breaking sustainability...
25 July 2024 1,545 3 View
Hello!!! I want to implement the Swerling characteristics functions (CF) directly in MATLAB without using its Fourier integral pairs...the Swerling CFs are actually Laplace Transform of the signal...
23 July 2024 4,925 1 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View