Which levels of zebrafish brain are usually analysed in which orientation when investigating abnormal growth?

24 November 2022 0 2K Report

I'm planning to perform immunohistochemical analysis on adult zebrafish brain slices. Is there are standard for the orientation of the brain slices? I found an article where they analysed 14 transversal slices across the whole brain, in another article 3 transversal, 2 parasagittal, 2 coronal. Does the selection of the orientation and level always depend on the aim of the experiment or are there also standards, when investigating growth abnormalities?

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