I have white wasabi meristems that are not dead but they are not green and shooting either. Meristems were placed on media with streptomycin for 5 days under darkness to reduce phenolic compound exudate and endogenous bacteria. 30% remained green chlorophyll 70% white as snow. The good news is there was no phenolic exudate and no bacteria.
The bad news is that there is no growth.
Can anyone explain why this is so and can this be reversed to show green again.
Hi
Exposing the cultures on streptomycin for more time will leads to bleaching of meristems. Instead use antibiotics during sterilization procedure.
Dehydrate or just leave the meristems for 2-3 days and then use them. This will reduce phenolics.
Hope this info will helps you...
Thanks for confirming my suspicion. Is this reversible? ie by placing on hormone free media etc.
You are welcome.
Just leave the ex-plants at room temperature without using any media for 2-3 days and then use for the experiments. This will dehydrate them and reduce the phenolic exudation.
Note: It worked for me but response differ from plant to plant. Try it on some explants.
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