We transfected AML cell line KG1 with the reagent lipofectamine LTX 300, and noticed some cells with the same shape, but twice bigger than usual. Maybe someone had also such experience, and could answer this question?
Successful transfection is influenced by many factors—the choice of the transfection method, health and viability of the cell line, number of passages, degree of confluency, quality and quantity of the nucleic acid used, and the presence or absence of serum in the medium can all play a part in the outcome of your transfection experiment.