DNA extraction
It is not 25:1 it is 24:1
What could be the possible reason for not getting proper band of ladder while performing PAGE
07 June 2023 7,641 1 View
What will be the condition that s11 will be the same? I have tried to replace copper with graphene, but the results are very different. I also changed the chemical potential from 0.1eV to 0.5ev,...
24 May 2023 5,804 5 View
How can we make a pin diode in ON/OFF state based on the values of RLC. I have studied that in ON state RLC operates in series and in OFF state it operates in parallel circuit. I am attaching the...
07 March 2023 7,784 7 View
How do we maintain the HPLC column usage log? Except excel and smart sheets, are ther any alternatives?
17 January 2023 8,532 4 View
Is it possible that ZnO/CNT nanocomposite does not show supercapacitor applications?
02 January 2023 455 5 View
I designed an antenna by randomly selecting the L-shaped slot on the patch and ground plane. But is there any method by which we can select a specific shape of the slot to obtain multiband...
17 December 2022 6,995 7 View
Group delay is used to measure the response in the ultra-wideband region. Can it be a good parameter to analyse the performance of a narrowband antenna? If yes, how? Please share your views on the...
01 September 2021 7,894 7 View
What will be the direction of electrons/holes in the conduction/valance band of HIT or Carrier Selective Contact (MoOx and NiOx) based Si solar cell for diffusion-limited/thermionic charge...
23 March 2021 3,257 3 View
There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...
01 March 2021 1,069 7 View
I am attaching my design and result of Gains.S11 parameter is less than -10dB from 3.1 to 9.8 Ghz.Z11 magnitude also lies between 40 -60 ohm for this range .Still gain is negative. Pls help
29 January 2021 4,630 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
