It is published somewhere that the addition of protein (often 0.1% BSA) is helpful for the right preservation of an aliquot of growth factor or cytokine?
Thanks a lot
BSA is a carrier or filler protein, that will prevent low level binding of the aliquoted growth factor/cytokine to the storage container and prevent inactivation, while under frozen conditions
Albumin is a natural carrier protein for many growth factors in the circulation. For a purified growth factor or cytokine it will prevent precipitation of the pure protein in a watery solution, as well as sticking to the carrier vessel by hydrophobic interactions.
You can consider DMSO as an alternative, if you want to avoid BSA. Higher growth factor/cytokine protein stock concentrations ( ~ 1 microgram / microliter ) can reduce a significant loss.
Dear All,
I have a relevant question. I found the background signal with 0.1% BSA also. Did you also observe the same thing?
Regards,
Nasir
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