For bone marrow trephines we use a glutaraldehyd fixative, which for internal reasons is imperative. Decalcification in EDTA has been changes from overnight at 35°C to a stirred bath at 45°C, but that didn't change anything
Why are you looking for CD56 in plasma cells?
It is rather in NK and in myoblasts.
try using purified NK cells to test the Ab for CD56
in our hands most Anti CD56 are better for FACS
I guess that this may be due to the glutaraldehyde fixation you are using. This can lead to a loss of antigenicity which may be more pronounced than that observed with formalin. Consider switching to formalin fixation for your bone arrow trephines. If that's not possible, try shorter fixation or test different antigen retrieval methods.
I take it, the stains you mentioned work well on standard FFPE tissues?
Hope this is helpful,
Good luck.
CD56 is only positive on malignant plasma cells of myeloma
CD138 and CD38 staining could be affected by the decalcification.
Firstly, we use use formalin for fixation.
Secondly, no IHC marker is 100% consistent. It does depend on the maturity of the cell in question.
Today I leave from the house and I shall be away from February, 20th till March, 26th, 2015
Thank you very much.
Yes, neoplastic plasma cells were our aim.
No, switching to formalin for internal reasons not the way we can go.
CD138 has been inconsistent in past years, that's we we had tried CD56.... A new batch now works very consistently. So we will stick to this for the next months. Thanky again!
Another sugggestion would be to use MUM1 as a marker for plasmacytoid differentiation. In our hands that works quite well in bone marrow trephines.
Vielen Dank Herr Niedobitek, schön auf diesem Weg mal wieder von Ihnen gehört zu haben. Haben uns mal früher beim Besuch in Dresden (mit Thomas Luther, Heinz Fehrenbach) kennen gelernt....
Ich erinnere mich. Ist ja schon lange her. Ich hoffe, es geht Ihnen in Köln gut.
Herzlchen Gruß
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