How to get a quantitative phase analysis from small amounts of sample?

Hello, Due to project internal reasons I can only obtain samples (multi-oxide systems) having a volume being to small for a quantitave XRD analysis. Is it correct to fill up the sample with a...

15 January 2021 8,389 5 View

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I am looking for high purity calcium phosphate powders. In order to fulfill a special application, it is necessary to obtain sintered bulks being white, but not blue. can anybody help me? Thank...

10 May 2020 1,295 3 View

Is there a free or low cost software for editing chromatograms?

For example I want to compare, align and edit forward and reverse chromatograms (by changing, deleting, inserting bases etc. within the chromatograms) and finally get the consensus sequence in...

26 June 2019 7,103 3 View

Protocols for determining live cells grown on transwell filters? Dye? LDH?

11 October 2016 4,436 3 View

Any differences in ecology and distribution Eleusine indica and E. Eleusine tristachya in Europe?

hallo, I need some information about the occurrence and distribution of Eleusine in (South-) Europe (Mediterranean, Spain/Iberia) incl. Canary Islands. Are there distribution maps? What are the...

30 October 2014 3,447 5 View

Selection of MSCs/ECFCs with puromycin after transduction - can anyone help?

12 March 2014 663 1 View

How does the distance between the layers in bilayer graphene vary with perpendicular pressure?

I wanted to calculate the change in distance "d" between the two layers in bilayer graphene for a given pressure "p" perpendicular to the surface. For continuous materials the relation between...

13 February 2013 619 4 View

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02 March 2021 3,060 3 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View