I purified my DNA sample from pcr product and initially it showed 43.8ng/microliters but after concentrating the DNA spectrophotometric measurement showed only 34.2 Ng/microliters. What should be the cause? How can I solve it?
DNA binds to standard plastic, e.g. polypropylene or polystyrene. This is especially evident when the DNA is at low abundance. I would suggest trying low DNA-binding tubes.