I am using DMSO as a negative control in an experiment being performed to measure calcium dynamics through GCaMP (GFP) fluorescence. DMSO is acting as a solvent for my synthetic organic compound supposed to change the calcium dynamics in cells. The problem is that DMSO is showing a considerable fluorescence signal. Based on the suspicion that our lab's DMSO is somehow contaminated, I borrowed some DMSO from a neighboring lab, but it also showed the signal of considerable fluorescence.
How can I solve this problem??
Hi Waheed Ahmed
What is the compound you need to dissolve in DMSO? Is it like a DREADD activator such as CNO or related molecules?
If yes, you could buy a dihydrochloride (i.e. water-soluble) version of the molecule. These are available for CNO for instance.
@ Vincent Simon
Compound is a derivative of Indole. It is supposed to activate GPCR like receptor as an agonist.
- Badges
- Science method