Waheed Ahmed

2 Questions 3 Answers 0 Followers

Questions related from Waheed Ahmed

Why is DMSO (negative control) showing a considerable signal of fluorescence?

I am using DMSO as a negative control in an experiment being performed to measure calcium dynamics through GCaMP (GFP) fluorescence. DMSO is acting as a solvent for my synthetic organic compound...

25 April 2023 3,031 5 View

Does the presence of restriction enzymes in plasmid/insert solutions interfere with the ligation reaction?

I digested my inserts with restriction enzymes and then purified them through columns. For the absorbance ratio (260/280), Nanodrop gave the value of 1.59, which indicates the presence of proteins...

18 October 2022 3,133 5 View