Lamp-1 is mainly located in the lysosomal membrane. When there is no other reference protein as a cell location, it appears to be in the cytoplasm. It is recommended to observe the fluorescence of lysosomal membrane-labeled proteins at the same time to better locate Lamp-1

Hi Akram, this is an interesting question. I have studies this little bit in the past. The overexpression of fluorescently labelled LAMP-1 can produce so much background during the fluorescence imaging. Typically you may have less than 2% of LAMP-1 in cytosol (or outside of the lysosme area) in normal cells.

If you have sometime, please have a look at this article.

Abeywickrama, C.S., Wijesinghe, K.J., Stahelin, R.V. and Pang, Y., 2019. Lysosome imaging in cancer cells by pyrene-benzothiazolium dyes: An alternative imaging approach for LAMP-1 expression based visualization methods to avoid background interference. Bioorganic chemistry, 91, p.103144.

Thanks

Chathura S. Abeywickrama , Thanks,

this was a very interesting paper. I could not find the vendor for pyrene-benzothiazolium dyes. could you please tell me where I can look at the product?