8 Questions 18 Answers 0 Followers
Questions related from Akram Etemadi
Hi everyone I am trying to coat a glass-bottom dish with Pam3CSK4 and plate THP-1 cells to measure IL6 with ELISA from the supernatant. I wondered if anyone knows a good concentration and...
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Hi every one I transfected Lamp-1 GFP plasmid to the HEK293 cell line. When I image the cells in TIRF, I see green fluorescence o the cytosol too. Does anyone have any idea that how it can...
22 December 2020 10,128 3 View
Hi every one I have made a cell line of HEK293 transfected LAPM-1GFP and after seeding the cells for 24h, I try to load dextran on the dish but after imaging, the cells with GFP+ have loaded less...
15 December 2020 1,945 2 View
Hi all, I am trying to measure the beta-hexosaminidase release in HEK-293 cells expressing recognition receptors after stimulating them I have tried p-NAG (4-Nitrophenyl N-acetyl-β- D...
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Hi Everyone, I tried to have the Alkanethiol as the SAMs on a nano-gold dots on silicon substrate. to make sure that the thiols are bond on the Au not Si, I used XPS to analyse the surface. The...
12 June 2019 3,187 10 View
Hi everyone I have made some gold nano-structures on Silicon and I want to bind Alkanethiols on the gold dots. I have to make sure they only bind on the gold and need to characterize them. I have...
30 April 2019 5,812 2 View
Hi everyone, I am trying to see Calcium influx in HEK-293 cells. I treat them with 1uM Fluo-4 and 1uM Cell tracker for 1 hour in 37C, then washing with DMEM, then keep them in incubator for 30 min...
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Hi, I am working with HEK293 expressed by Dectin-1. I have a problem after fixation with PFA 4%. despite the fact that I am coating the cover glasses with PLL, the cells jump off after fixation. I...
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