19 Questions 22 Answers 0 Followers
Questions related from Akram Etemadi
Hi every one I want to coat the 35mm matTek dish with streptavidin, I have 2 questions: 1- can I resolve the streptavidin in water instead of PBS for coating? 2- how long is enough to coat the...
27 January 2021 3,916 0 View
Hi everyone I am trying to coat a glass-bottom dish with Pam3CSK4 and plate THP-1 cells to measure IL6 with ELISA from the supernatant. I wondered if anyone knows a good concentration and...
22 January 2021 2,994 1 View
Hi I want to have a positive control for lysosomal exocytosis in HEK293 cells. I treat the cells with Streptolysin O for 1Unit, 0.5 Unit and did not see any lysosome exocytosis on TIRF. I think...
07 January 2021 1,671 0 View
Hi every one I transfected Lamp-1 GFP plasmid to the HEK293 cell line. When I image the cells in TIRF, I see green fluorescence o the cytosol too. Does anyone have any idea that how it can...
22 December 2020 10,381 3 View
Hi every one I have made a cell line of HEK293 transfected LAPM-1GFP and after seeding the cells for 24h, I try to load dextran on the dish but after imaging, the cells with GFP+ have loaded less...
15 December 2020 2,163 2 View
Hi all I am trying to assay for beta-hexosaminidase release with ( 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide β-N-acetylhexosaminidase substrate) for HEK-293 cells expressing Dectin-1....
09 January 2020 8,216 0 View
Hi all, I am trying to measure the beta-hexosaminidase release in HEK-293 cells expressing recognition receptors after stimulating them I have tried p-NAG (4-Nitrophenyl N-acetyl-β- D...
17 November 2019 6,451 1 View
Hi Everyone. I have made the nanofabricated TEM window grid including gold dot arrays on Si3N4 grids. I tried to generate SAMs by immersing the grid on the solution of (biotinylated Alkanethiols)...
05 November 2019 3,337 0 View
Hi every one I am looking for a Silane PEG conjugated product with Atto 488 and also Texas red, I am wondering if I can find them commercially or if not how I can prepare them using Silane-PEG...
30 September 2019 9,260 0 View
Hi Everyone, I tried to have the Alkanethiol as the SAMs on a nano-gold dots on silicon substrate. to make sure that the thiols are bond on the Au not Si, I used XPS to analyse the surface. The...
12 June 2019 3,279 10 View
Hi everyone I have made some gold nano-structures on Silicon and I want to bind Alkanethiols on the gold dots. I have to make sure they only bind on the gold and need to characterize them. I have...
30 April 2019 5,890 2 View
Hi every one, I am making very small nano-fabrication patterns over negative photoresist, like 200 nm pitch size. After etching the icon7, the diameter of the holes in the 2D pattern will get...
21 January 2019 7,698 3 View
Hi everyone. I am gonna use an Acid Sphingomyelinase inhibitor to inhibit ASM and see the its effects on endocytosis of a receptor. I am using 1-Aminodecylidene bis-phosphonic acid that is...
27 August 2018 6,500 9 View
Hi Every one, I am doing some IF experiments to see the early endosome after Dectin-1 activation by glucan. I am using Alexa 647 to tag Dectin-1 and EEA1 for endosome. I have two channels in...
08 May 2018 1,757 0 View
Hi everyone, I am trying to see Calcium influx in HEK-293 cells. I treat them with 1uM Fluo-4 and 1uM Cell tracker for 1 hour in 37C, then washing with DMEM, then keep them in incubator for 30 min...
04 April 2018 6,976 25 View
Hi, I am working with HEK293 expressed by Dectin-1. I have a problem after fixation with PFA 4%. despite the fact that I am coating the cover glasses with PLL, the cells jump off after fixation. I...
09 October 2017 2,675 2 View
Hi I am trying to form SAMs on a gold film. I am using Alkynthiols with S-H terminal. I am wondering if it is necessary to hydrogenate the surface to make a better quality of Sulfur-Au bond? Thanks
01 January 1970 4,519 0 View
01 January 1970 1,385 0 View
Hi all I am binding Streptavidin on biotinylated self-assembled monolayers (10% final concentration 1mM) on gold arrays. afterward, I bind biotinylated docking strand DNA on streptavidin. I am...
01 January 1970 7,399 0 View
