Asavari Ani Joshi

4 Questions 21 Answers 0 Followers

Questions related from Asavari Ani Joshi

Is it a good idea to change the maturation media for 3T3 L1 differentiation every day as media is looking very acidic?

I am differentiating 3T3 L1 cells to adipocytes. The maturation medium containg (DMEM high glucose+FBS 10%+ insulin 10µg/ml) is very acidic. majority of protocols mention chaning medium after 2...

05 May 2017 802 15 View

Which are the cell lines used in study for wound healing process?

we are keen to evaluate certain molecules for their potential to accelerate wound healing process in in vitro set up. Two cell culture models i.e 3T3 and hPBMCs we are using. I would like to...

05 May 2016 6,026 7 View

Why freezing mixture not getting frozen even at -80?

Recently we are facing this problem. We are using 20%complete medium + 10%DMSO or 90%FBS+ 10%DMSO as freezing mixture. None of these freezing mixtures are freezing even at -20. we are sure our...

05 May 2016 3,206 6 View

What are the markers for separating oral SCC cells (from patients) from normal cell?

03 March 2016 4,612 3 View