Why does using two different programs to assemble sanger forward and reverse sequences result in two different consensus sequences?

29 April 2023 1 10K Report

I used Geneious and codoncon Aligner programs to assemble my reads and I noticed that there was a slight difference in at least the beginning.

Katie A S Burnette

It's probably a difference in the quality cut-off by the different programs. The first ~50 bases are normally low-quality and will be trimmed off. The exact threshold varies by program.

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