When i checked the interaction using dimplot, no protein-protein interaction but ligplot detected it as ligand.
Hi all. I ran the enzyme activity (pure Tyrosinase after purification using recombinant e coli) using L-Dopa with this condition 50 mM phosphate buffer ph 6, 1mM L-Dopa 25 C temperature 0.5 mM...
06 February 2024 8,350 4 View
I am a bit confused about the matter. The majority of papers and explanations I have come across indicate that the inhibited line is generally positioned above the uninhibited line, but I have...
31 May 2023 3,098 1 View
I have done protein -protein docking using hdock server. however the interaction cannot be viewed using ligplot as shown in the attached photo. does anyone has the same experience? Thanks !
11 December 2022 4,158 5 View
Hi all, Im using HDOCK server to see protein-protein binding, I got the 10 best predictions and downloaded the pdb files. However when I open it to see the interaction using Ligplot, it failed and...
25 November 2022 3,621 2 View
Hi, I am currently calculating my ic50 using graphpad, however im confused whether to use my data from the raw data (triplicates) of each concentration or from the mean ? my ic50 is based on the...
27 February 2022 9,074 4 View
Im doing enzyme assay for the proteases of sars cov 2 . However with different concentrations of the protein, the solution did not change colour to yellow. Im wondering if these substrates have...
11 November 2021 4,484 10 View
In cyclic voltammogram there are two region one is faradic and other is capacitive region. So If we know our Open circuit voltage (OCV) and we are restricting our study of Cyclic voltammetry...
08 March 2021 2,078 3 View
i want to do GCD study of specific material in a specific potential let discharge from 1.0V to 0.2V and again Charge it to 1.0V. How to perform this experiment using pine instrument?
03 January 2021 10,112 1 View
I want to gather feedback from the implementation of processes in order to optimize them and I was wondering what tools other than 'google forms' could allow me to implement them in a formal and...
23 December 2019 8,570 3 View
I would like to figure out how can I create BPMN process's relationships within my process repository The idea is to have a Parent process with for example Task A, Task B and Task C. Then I made...
24 November 2019 7,716 0 View
The paper in question is "Interpolation of Nitrogen Fertilizer Use in Canada from Fertilizer Use Surveys". This paper was very recently published by Agronomy (MDPI). Agronomy has, in the last day...
07 August 2024 9,934 3 View
Program: g_mmpbsa, version 2024.1 Source file: extrn_apbs.cxx (line 152) Fatal error: Failed to execute command: $APBS pybYcUWA.in --output-file=pybYcUW.out
07 August 2024 6,066 0 View
The first pdf file I uploaded had an error. So I uploaded an updated, corrected pdf of that paper with a different pdf name. I dpon't want the old copy to be download or read.
07 August 2024 9,508 1 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Dear Researchers Kindly share JCPDS 65-7246 file Thanks in advance
04 August 2024 5,613 1 View
Hi everyone I need a file with a dirty and clean potato image
04 August 2024 7,199 4 View
why don't d-orbitals split themselves because of themselves without the presence of ligands? Electrons are indistinguishable. Why wouldn't it be more correct that protons from a ligand split the...
03 August 2024 3,589 3 View
I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...
03 August 2024 1,370 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View