Haslina Asis

6 Questions 13 Answers 0 Followers

Questions related from Haslina Asis

Why does Tyrosinase suddenly become inactive when it was active previously?

Hi all. I ran the enzyme activity (pure Tyrosinase after purification using recombinant e coli) using L-Dopa with this condition 50 mM phosphate buffer ph 6, 1mM L-Dopa 25 C temperature 0.5 mM...

07 February 2024 8,218 4 View

What does it mean if inhibited line below the uninhibited line in lineweaver burk plot?

I am a bit confused about the matter. The majority of papers and explanations I have come across indicate that the inhibited line is generally positioned above the uninhibited line, but I have...

01 June 2023 3,064 1 View

Why does hdock file cannot be viewed using ligplot for protein-protein interaction?

I have done protein -protein docking using hdock server. however the interaction cannot be viewed using ligplot as shown in the attached photo. does anyone has the same experience? Thanks !

12 December 2022 4,124 5 View

How to see Interaction using Ligplot after using Hdock server?

Hi all, Im using HDOCK server to see protein-protein binding, I got the 10 best predictions and downloaded the pdb files. However when I open it to see the interaction using Ligplot, it failed and...

26 November 2022 3,587 2 View

Ic50 using graphpad prism?

Hi, I am currently calculating my ic50 using graphpad, however im confused whether to use my data from the raw data (triplicates) of each concentration or from the mean ? my ic50 is based on the...

28 February 2022 9,033 4 View

WHY THE FRLKGG-pNa (susbtrate for PLPRO) and Z-TSAVLQ-pNa (substrate for MPRO) DO NOT CHANGE COLOUR DURING ENZYME ASSAY?

Im doing enzyme assay for the proteases of sars cov 2 . However with different concentrations of the protein, the solution did not change colour to yellow. Im wondering if these substrates have...

12 November 2021 4,456 10 View